Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

96.6K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
96.6K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.1K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.1K
SDS-PAGE01:27

SDS-PAGE

23.3K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
23.3K
Electrophoresis: Overview01:20

Electrophoresis: Overview

4.2K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.2K
Southern Blot02:57

Southern Blot

15.1K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
15.1K
DNA Isolation01:34

DNA Isolation

177.4K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
177.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arginine-Derived Cationic Surfactants Containing Phenylalanine and Tryptophan: Evaluation of Antifungal Activity, Biofilm Eradication, Cytotoxicity, and Ecotoxicity.

Journal of xenobiotics·2025
Same author

Insights on Microplastic Contamination from Municipal and Textile Industry Effluents and Their Removal Using a Cellulose-Based Approach.

Polymers·2024
Same author

Liquid-liquid phase separation (LLPS) in DNA and chromatin systems from the perspective of colloid physical chemistry.

Advances in colloid and interface science·2024
Same author

Effect of polymer addition on the phase behavior of oil-water-surfactant systems of Winsor III type.

Physical chemistry chemical physics : PCCP·2023
Same author

Size Matters? A Comprehensive In Vitro Study of the Impact of Particle Size on the Toxicity of ZnO.

Nanomaterials (Basel, Switzerland)·2023
Same author

Melanogenesis and Hypopigmentation: The Case of Vitiligo.

Indian journal of dermatology·2023

Related Experiment Video

Updated: May 7, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

11.2K

DNA gel particles: an overview.

M Carmen Morán1, M Pilar Vinardell1, M Rosa Infante2

  • 1Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII, 08028 Barcelona, Spain; Interaction of Surfactants with Cell Membranes, Unit Associated with CSIC, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXII, 08028 Barcelona, Spain.

Advances in Colloid and Interface Science
|October 15, 2013
PubMed
Summary

Researchers developed novel DNA gel particles for controlled release applications. Varying cationic agents influences particle properties and DNA release, enabling potential therapeutic uses.

Keywords:
CytotoxicityDNA gelsDNA releaseDNA–oppositely charged agent interactionHemocompatibilityParticles

More Related Videos

Denaturing Gradient Gel Electrophoresis DGGE
10:52

Denaturing Gradient Gel Electrophoresis DGGE

Published on: February 25, 2007

21.3K
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.2K

Related Experiment Videos

Last Updated: May 7, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

11.2K
Denaturing Gradient Gel Electrophoresis DGGE
10:52

Denaturing Gradient Gel Electrophoresis DGGE

Published on: February 25, 2007

21.3K
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.2K

Area of Science:

  • Materials Science
  • Biotechnology
  • Polymer Chemistry

Background:

  • Understanding DNA interactions with charged compounds is key for novel DNA-based materials.
  • DNA gel particles can be engineered for controlled release of single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA).

Purpose of the Study:

  • This review synthesizes recent advancements in DNA gel particles formed at water-water emulsion interfaces.
  • It examines how cationic agents affect DNA entrapment, particle morphology, swelling, dissolution, and DNA release.

Main Methods:

  • The review analyzes studies focusing on DNA gel particle formation and characterization.
  • It discusses the impact of different cationic cosolutes on gelation and particle properties.
  • Therapeutic applications are explored through assessments of surface hydrophobicity, hemolysis, and cytotoxicity.

Main Results:

  • The chemical structure of the cationic cosolute dictates association strength and gelation homogeneity.
  • DNA gel particle properties like entrapment, morphology, and swelling are tunable via the cationic agent.
  • Recent studies assess the suitability of these DNA gel particles for therapeutic applications.

Conclusions:

  • DNA gel particles offer a versatile platform for controlled DNA release, with properties modulated by cationic agent choice.
  • These materials show promise for therapeutic applications, pending further investigation into their biological interactions.