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Acid secretion and intracellular pH in isolated oxyntic cells
The Journal of Membrane Biology
|January 12, 1978
Summary
Isolated oxyntic cells secrete hydrochloric acid when stimulated. This study reveals a neutral anion exchange mechanism controlling acid-base balance in these cells.
Area of Science:
- Physiology
- Cell Biology
- Gastroenterology
Background:
- Oxyntic cells are crucial for gastric acid secretion.
- Understanding the regulation of hydrochloric acid (HCl) secretion is vital for gastrointestinal health.
Purpose of the Study:
- To investigate the capacity of isolated oxyntic cells to secrete HCl.
- To elucidate the mechanisms underlying HCl secretion and intracellular pH regulation.
Main Methods:
- Stimulation of isolated oxyntic cells with various secretagogues.
- Measurement of medium acidification and intracellular pH using the DMO14C technique.
- Assessment of inhibitor effects on acid secretion and cell pH.
Main Results:
- Isolated oxyntic cells demonstrated HCl secretion upon stimulation, with transient acidification peaks.
- Stimulation with dibutyryl cyclic AMP (db-cAMP) increased cell pH by 0.13 units.
- Acid secretion and pH changes were blocked by specific inhibitors and SCN- pretreatment.
- Calculated intracellular base concentration increased by 5-10mM upon stimulation.
Conclusions:
- Isolated oxyntic cells actively secrete hydrochloric acid.
- A neutral anion exchange mechanism at the serosal membrane likely regulates intracellular acid-base balance during stimulation.