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Updated: Jul 25, 2026
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
Published on: May 1, 2014
[Isolation, culture and identification of human odontoclasts]
Wei Zhao1, Jiao-cui Wang, Xiao-ying Chen
1Department of Pediatric Dentistry, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
Objective:
To isolate, culture and identify odontoclasts in vitro and to establish a method of culturing human odontoclasts.
Methods:
Healthy and retentive deciduous teeth were extracted, and then placed in α-minimum essential medium containing 0.1% collagenase and 0.2% dispase for 1 h.Odontoclasts were obtained and incubated from the absorbing root surfaces of deciduous teeth.Isolated cells were viewed by inverted phase contrast microscope firstly. Then, the isolated odontoclasts were morphologically observed by hematoxylin and eosin staining (HE) and tartrate-resistant acid phosphatase (TRAP) staining. The prepared teeth slices were cocultured with the isolated odontoclasts and scanning electronic microscope(SEM) was used to demonstrate the presence of resorption lacunae.
Results:
The isolated odontoclasts appeared as multinucleated giant cell with many vacuolus in cytoplasm. TRAP staining demonstrated that the cytoplasm of the odontoclasts was full of claret-red positive particles.Resorption lacunae on teeth slices which cocultured with odontoclasts were seen under SEM.
Conclusions:
Enzyme digestion is an effective method to isolate odontoclasts from absorbing root surface of deciduous teeth.
