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Updated: May 7, 2026

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
Published on: March 9, 2018
[Aspergillus species in hospital environments with pediatric patients in critical condition]
Mariana Fernández1, María Cattana1, Florencia Rojas1
1Departamento de Micología, Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Argentina.
Background:
Aspergillus is a group of opportunistic fungi that cause infections, with high morbimortality in immunosuppressed patients. Aspergillus fumigatus is the most frequent species in these infections, although the incidence of other species has increased in the last few years.
Aims:
To evaluate the air fungal load and the diversity of Aspergillus species in hospitals with pediatric patients in critical condition.
Methods:
The Intensive Care Unit and Burns Unit of a pediatric hospital were sampled every 15 days during the autumn and spring seasons. The air samples were collected with SAS Super 100(®) and the surface samples were collected by swab method.
Results:
The UFC/m(3) counts found exceeded the acceptable levels. The UFC/m(3) and the diversity of Aspergillus species found in the Intensive Care Unit were higher than those found in the Burns Unit. The fungal load and the diversity of species within the units were higher than those in control environments. The use of both methods -SAS and swab- allowed the detection of a higher diversity of species, with 96 strains of Aspergillus being isolated and 12 species identified. The outstanding findings were Aspergillus sydowii, Aspergillus niger, Aspergillus flavus, Aspergillus terreus and Aspergillus parasiticus, due to their high frequency. Aspergillus fumigatus, considered unacceptable in indoor environments, was isolated in both units.
Conclusions:
Aspergillus was present with high frequency in these units. Several species are of interest in public health for being potential pathogenic agents. Air control and monitoring are essential in the prevention of these infections.
Insights
Air fungal contamination by Aspergillus species is high in pediatric critical care units. Monitoring and control are essential to prevent infections in vulnerable patients.
Area of Science:
- Mycology
- Environmental Microbiology
- Hospital-Acquired Infections
Context:
- Aspergillus species are opportunistic fungi causing severe infections in immunocompromised individuals.
- An increasing incidence of non-fumigatus Aspergillus species has been observed in clinical settings.
- Pediatric patients in critical condition are particularly susceptible to fungal infections.
Purpose:
- To assess airborne fungal load and Aspergillus species diversity in pediatric intensive care and burns units.
- To compare fungal contamination levels between different critical care environments.
- To identify prevalent Aspergillus species posing potential public health risks.
Summary:
- Air sampling in pediatric Intensive Care and Burns Units revealed fungal contamination exceeding acceptable levels.
- A high diversity of Aspergillus species, including potential pathogens like Aspergillus sydowii, niger, flavus, terreus, and parasiticus, was identified.
- Aspergillus fumigatus was detected in both units, highlighting the need for stringent environmental controls.
Impact:
- Findings underscore the significant presence of Aspergillus in critical pediatric environments.
- Identified species highlight potential public health concerns and the need for targeted interventions.
- Emphasizes the critical role of continuous air monitoring and control strategies in preventing invasive aspergillosis.
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