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Alpha-naphthylisothiocyanate modulates hepatobiliary transporters in sandwich-cultured rat hepatocytes

Cen Guo1, Lei He, Dan Yao

  • 1Center for Drug Safety Evaluation & Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.

Toxicology Letters
|October 15, 2013
PubMed

Keywords:
5 (and 6)-carboxy-2′,7′-dichlorofluorescein5 (and 6)-carboxy-2′,7′-dichlorofluorescein diacetateALTANITAlpha-naphthylisothiocyanateBECsBEIBsepCDFCDFDACl(biliary)Cyp27a1Cyp7a1Cyp8b1DPDPEDrug-induced cholestasisELISAGC–MSHBSSHank's balanced salt solutionIPRLsLC–MS/MSLDHMrpNtcpOatpPLS-DASCRHSandwich-cultured hepatocytesTransporterVPA[D-Pen(2,5)]-enkephalinalanine transaminasealpha-naphthylisothiocyanatebile salt export pumpbiliary clearancebiliary epithelial cellsbiliary excretion indexcholesterol 7α-hydroxylased8-TCAdeuterium-labeled sodium taurocholateenzyme-linked immunosorbent assaygas chromatography–mass spectrometryisolated perfused rat liverslactate dehydrogenaseliquid chromatography–mass spectrometry tandem mass spectrometrymultidrug resistance-associated proteinorganic anion transporting polypeptidepartial least squares discriminant analysissandwich-cultured rat hepatocytessodium/taurocholate cotransporting polypeptidesterol 12α-hydroxylasesterol 27-hydroxylasevalproic acid

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