Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.4K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.4K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.0K
5.0K
DNA Microarrays02:34

DNA Microarrays

16.9K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
16.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Midline signaling regulates kidney positioning but not nephrogenesis through Shh.

Developmental biology·2010
Same author

Y chromosomal STR polymorphism in northern Chinese populations.

Biological research·2010
Same author

Construction of NF-κB-targeting RNAi adenovirus vector and the effect of NF-κB pathway on proliferation and apoptosis of vascular endothelial cells.

Molecular biology reports·2010
Same author

Hearing evaluation of intratympanic methylprednisolone perfusion for refractory sudden sensorineural hearing loss.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2010
Same author

Hydrothermal synthesis of nanostructures Bi12TiO20 and their photocatalytic activity on acid orange 7 under visible light.

Chemosphere·2010
Same author

An anticancer drug delivery system based on surfactant-templated mesoporous silica nanoparticles.

Biomaterials·2010
Same journal

TBX6 promotes proliferation, invasion, and migration in colorectal cancer: Integrated transcriptomic and protein interaction network analysis.

Gene·2026
Same journal

Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

Gene·2026
Same journal

Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

Gene·2026
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

23.6K

Selected isomiR expression profiles via arm switching?

Li Guo1, Hui Zhang, Yang Zhao

  • 1Department of Epidemiology and Biostatistics and Ministry of Education Key Lab for Modern Toxicology, School of Public Health, Nanjing Medical University, 211166, China.

Gene
|October 15, 2013
PubMed
Summary
This summary is machine-generated.

Both arms of microRNAs (miRNAs) can produce functional molecules. This study compares miRNA isomiR expression from both arms, revealing diverse profiles and influencing factors like arm switching.

Keywords:
3′ UTR3′-untranslated regionsArm switchingHomo sapiensIsomiRIsomiR expression profileKSKolmogorov–SmirnovMicroRNA (miRNA)Mus musculusRISCRNA-induced silencing complexSus scrofahsamiRNAmiRNA starmiRNA*microRNAmmupre-miRNAprecursor miRNApri-miRNAprimary miRNAssc

More Related Videos

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.6K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

8.2K

Related Experiment Videos

Last Updated: May 7, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

23.6K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.6K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

8.2K

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Mature microRNAs (miRNAs) are typically derived from either the 5p or 3p arm of a hairpin precursor.
  • Evidence suggests both arms of pre-miRNAs can yield functional mature miRNAs, challenging the traditional view of exclusive arm selection.
  • The phenomenon of 'arm switching' allows for flexible miRNA generation, adding complexity to miRNA biogenesis.

Purpose of the Study:

  • To compare the isomiR expression profiles between the 5p and 3p arms of pre-miRNAs.
  • To investigate the conservation and diversity of isomiR expression patterns across different samples and conditions.
  • To understand how factors like abundance and arm switching influence miRNA isomiR profiles.

Main Methods:

  • Analysis of in-house and published small RNA deep sequencing datasets.
  • Comparison of isomiR types and dominant isomiR species between miR-#-5p and miR-#-3p.
  • Assessment of isomiR profile conservation in 5' ends and enrichment levels.

Main Results:

  • Fewer miRNA pairs (11 in tumor, 6 in normal cells) were simultaneously identified as abundant species, despite many miR-#-5p and miR-#-3p being functional.
  • miR-#-5p and miR-#-3p exhibited varied isomiR expression profiles and diverse enrichment levels.
  • IsomiR profiles of non-dominant arms showed less conservation at the 5' end compared to dominant arms.
  • Abundantly co-expressed miR-#-5p and miR-#-3p displayed stable isomiR expression profiles across samples.
  • IsomiR expression patterns are influenced by 'arm switching' and contribute to the complexity of the regulatory network.

Conclusions:

  • Both miRNA arms can generate distinct and complex isomiR populations.
  • IsomiR diversity and abundance are influenced by factors including arm switching and cellular context.
  • The intricate isomiR landscape significantly contributes to the complexity of coding-non-coding RNA regulatory networks.