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Barbiturates decrease voltage-dependent calcium conductance of mouse neurons in dissociated cell culture.

M A Werz, R L Macdonald

    Molecular Pharmacology
    |September 1, 1985
    PubMed
    Summary
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    Barbiturates, like pentobarbital and phenobarbital, reduce neurotransmitter release by decreasing neuronal calcium entry. This action is likely due to enhanced calcium channel inactivation or open channel block.

    Area of Science:

    • Neuroscience
    • Pharmacology

    Background:

    • Barbiturates are known to inhibit neurotransmitter release.
    • This inhibition is hypothesized to involve reduced calcium influx into neurons.

    Purpose of the Study:

    • To investigate the mechanisms by which barbiturates affect neuronal calcium entry.
    • To elucidate the impact of specific barbiturates on calcium-dependent action potentials and currents.

    Main Methods:

    • Utilized mouse dorsal root ganglion neurons in cell culture.
    • Administered pentobarbital and phenobarbital.
    • Performed voltage-clamp studies and intracellular injections of cesium (potassium channel blocker).

    Main Results:

    • Barbiturates decreased the calcium-dependent component of action potentials and afterhyperpolarization.

    Related Experiment Videos

  • Voltage-clamp experiments showed barbiturates reduced inward calcium current and conductance.
  • Cadmium ions blocked calcium conductance, confirming barbiturate action on calcium channels.
  • Barbiturates accelerated calcium current inactivation.

    • Conclusions:

    • Barbiturates reduce neuronal calcium conductance.
    • This reduction is achieved by either enhancing calcium channel inactivation or inducing an open channel block.
    • Findings clarify barbiturate's mechanism of action on neurotransmission.