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Updated: May 7, 2026
Microfluidic Mixers for Studying Protein Folding
Published on: April 10, 2012
Real-time investigation of protein unfolding at an air-water interface at the 1 s time scale
Yohko F Yano1, Etsuo Arakawa, Wolfgang Voegeli
1Department of Physics, Kinki University, 3-4-1 Kowakae, Higashiosaka City, Osaka 577-8502, Japan.
Abstract:
Protein unfolding at an air-water interface has been demonstrated such that the X-ray reflectivity can be measured with an acquisition time of 1 s using a recently developed simultaneous multiple-angle-wavelength-dispersive X-ray reflectometer. This has enabled the electron density profile of the adsorbed protein molecules to be obtained in real time. A globular protein, lysozyme, adsorbed at the air-water interface is found to unfold into a flat shape within 1 s.
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