Related Experiment Video
Updated: May 7, 2026
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
Effects of botulinum toxin A on patient-specific keloid fibroblasts in vitro
Frank Haubner1, Michaela Leyh, Elisabeth Ohmann
1Laboratory of Facial Plastic Surgery Research, Department of Otorhinolaryngology, University of Regensburg, Germany.
Objectives/Hypothesis:
To test whether therapeutic effects of botulinum toxin A on patient- specific keloid tissue can be reproduced on the cellular level. Specifically, effects on cell proliferation and expression of growth factors and cytokines relevant for wound healing were to be tested.
Study Design:
Experimental study including patient specific cell cultures of keloids.
Methods:
Patient-specific keloid tissue was tested in a cell culture model for effects of botulinum toxin incubation on cell proliferation and expression of the following cytokines and growth factors: IL-6 (interleukin-6), VEGF (vascular endothelial growth factor), TGF-β (transforming growth factor-β).
Results:
None of the tested parameters of human keloid tissue were affected by botulinum toxin A incubation.
Conclusion:
The present study does not add evidence to suggest a significant therapeutic role of botulinum toxin injections for the treatment of keloids.
Level Of Evidence:
N/A.
