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Updated: Aug 22, 2026

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
Published on: December 3, 2007
Abstract:
Replication of two Tahyna virus strains in the Aedes albopictus mosquito cell line was studied in the temperature range from 6 to 28 degrees C. The virus grew in this temperature range; its replication rate was related to the temperature of incubation. At lower temperatures the virus titres increased more slowly and did not reach as high maximum values as at higher temperatures. Short increase in incubation temperature resulted in a short increase in the titre of virus previously incubated at 10 degrees C, but not of virus previously incubated at 15 degrees C. At 10 and 15 degrees C, the released virus was demonstrated for more than 300 days past infection (p.i.).

