Reaction-based and single fluorescent emitter decorated ratiometric nanoprobe to detect hydrogen peroxide

A novel reaction-based cross-linked polymeric nanoprobe with a self-calibrating ratiometric fluorescence readout to selectively detect H2O2 is reported. The polymeric nanoprobe is fabricated by using hydrophobic H2O2-reactive boronic ester groups, crosslinker units, and environmentally sensitive 3-hydroxyflavone fluorophores through a miniemulsion polymerization. On treatment with H2O2, the boronic esters in the polymer are cleaved to form hydrophilic alcohols and subsequently lead to a hydrophobic-hydrophilic transition. Covalently linked 3-hydroxyflavones manifest the change in polarity as a ratiometric transition from green to blue, accompanied by a 500-fold increase in volume. Furthermore, this nanoprobe has been used for ratiometric sensing of glucose by monitoring the H2O2 generated during the oxidation of glucose by glucose oxidase, and thus successfully distinguished between normal and pathological levels of glucose.