[Principles of self-adjusting staining of sections of cerebral cortex for automatic structural analysis]

The development of methods of an automatic structural analysis which uses modern methods of image processing has put forward requirements to standardize the staining of the preparations studied. However, the examination of autopsy material of the human brain shows that the standard procedure of staining does not always lead to standard results. The author presents a formalized description of changes in the quality of preparation staining at different stages, which is based on the measurement of distributions of the mean gradients of the section image obtained with the help of the textural analysis system TAS produced by Leitz. The procedure of adaptive staining based on this description is proposed, which will help to compensate for differences in tinctural properties of different samples of autopsy material.