Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cocktail-style multifunctional resuscitation fluids: Where are we now in 2026?

Journal of anesthesia and translational medicine·2026

Same author

Optimizing the clinical management of prostate cancer in Taiwan: A multidisciplinary consensus.

Journal of the Formosan Medical Association = Taiwan yi zhi·2026

Same author

A Novel Method to Generate Analyte-Specific Specimens for Multi-Omic Studies of Primary Bladder Cancer.

Biopreservation and biobanking·2026

Same author

Radiographically-Measured Sarcopenia Predicts Hospitalization in Patients With Crohn's Disease.

Journal of clinical gastroenterology·2026

Same author

Captopril restores microglial homeostasis and reverses ASD-like phenotype in a model of ASD induced by exposure in utero to anti-caspr2 IgG.

Molecular psychiatry·2025

Same author

Classic Imaging Signs in Hepatobiliary Radiology: A Multimodality Review.

Seminars in ultrasound, CT, and MR·2025

Search research articles

Related Experiment Video

Updated: May 7, 2026

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

Published on: July 21, 2009

Conditional prerequisites for microchannel cytologic analysis on wet mount (fluid-based) biopsies.

Wilfrido Mojica¹, Rosemary Bassey, Frank Chen

¹Department of Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, New York.

Cancer Cytopathology

|October 15, 2013

Summary

Microfluidic channels can process patient cells for both cytology and molecular analysis without significantly altering tumor cell features. This approach integrates diagnostic and molecular assays for small biopsies, with time affecting non-tumor cells more than shear stress.

Keywords:

anoikis cytology fluid-based biopsy fluid-shear stress microfluidic channel

More Related Videos

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies

Published on: September 29, 2009

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

Published on: July 9, 2014

Related Experiment Videos

Last Updated: May 7, 2026

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

Published on: July 21, 2009

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies

Published on: September 29, 2009

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

Published on: July 9, 2014

Area of Science:

Biomedical Engineering
Cell Biology
Genomic Medicine

Background:

Genomic sequencing is advancing towards clinical applications, posing challenges for specimen allocation in small biopsies.
Integrating traditional cytology with molecular analysis from a single specimen is crucial.
Microfluidics offers a potential solution for simultaneous cytologic and molecular analysis.

Purpose of the Study:

To evaluate the impact of microfluidic processing on cell morphology.
To determine if fluid-shear stress or anoikis alter cytologic features.
To assess the feasibility of microfluidics for integrated diagnostics.

Main Methods:

Cells were subjected to flow-through in a microfluidic channel.
Qualitative and quantitative evaluations assessed alterations from fluid-shear stress and anoikis.
Non-tumor and tumor cells were analyzed separately.

Main Results:

Microfluidic processing dispersed cell clusters into smaller groups and individual cells.
Quantitative analysis revealed increased nuclear parameter variation in non-tumor cells, but not tumor cells.
Time-dependent processing impacted non-tumor cell cytology more than fluid-shear stress.

Conclusions:

Microfluidic channel passage is a viable method for cell processing.
This technique can be integrated into platforms for combined cytologic assessment and downstream molecular assays.
Future platforms can leverage microfluidics for comprehensive analysis of patient specimens.