Influenza virus hemagglutinin-binding T cells in delayed hypersensitivity induced in mice

The ability of spleen T cells from mice inoculated with influenza virus to transfer delayed hypersensitivity increases after 5 days culture in the presence of TCGF and influenza virus or its hemagglutinin. Enriched T cells have also a greater ability to bind viral hemagglutinin than cells which were not cultured and restimulated with either antigen. The results suggest the selection in vitro of specific antigen-binding T cells, able to transfer delayed hypersensitivity.