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Multiplication in liquid medium of Treponema sp. isolated from intestinal contents of swine
Abstract:
Treponema hyodysenteriae and Treponema innocens were multiplied by a simple culture method in liquid medium. TSB medium was prepared by the PRAS method in plasma bottles containing glass beads. Spirochaetes were injected through the rubber stopper and the bottles were incubated while revolving round their axes. The most abundant growth of spirochaetes in rotary culture was observed after 72 h incubation at 40 degrees C. whereas the highest number of viable cells in stationary culture was observed after 120 h. However, in the latter case the number of cells was lower than introduced at inoculation. Growth of the bacteria was stimulated by equine serum and 5% addition of rumen fluid. Optimal growth temperature was 40 degrees C.
Insights
Researchers optimized a simple liquid culture method for multiplying Treponema hyodysenteriae and Treponema innocens. Rotary cultures at 40°C for 72 hours yielded the most abundant spirochaete growth.
Area of Science:
- Microbiology
- Veterinary Science
Background:
- Treponema hyodysenteriae and Treponema innocens are significant pathogens in swine.
- Efficient culture methods are crucial for research and diagnostics.
Purpose of the Study:
- To develop and optimize a simple liquid culture method for Treponema species.
- To determine optimal conditions for bacterial growth and multiplication.
Main Methods:
- Utilized a roll-tube method with TSB medium under anaerobic conditions (PRAS).
- Incubated cultures at varying temperatures and durations in both rotary and stationary setups.
- Assessed bacterial growth by monitoring viable cell counts.
Main Results:
- Optimal growth temperature for both species was determined to be 40°C.
- Rotary cultures achieved peak growth after 72 hours, while stationary cultures required 120 hours.
- Equine serum and 5% rumen fluid addition significantly stimulated bacterial growth.
Conclusions:
- A simple rotary culture method at 40°C for 72 hours is efficient for multiplying Treponema species.
- Supplementation with equine serum and rumen fluid enhances bacterial yield.
- This optimized method facilitates further research on these important swine pathogens.