Isolation and characterization of chromatin from Caulobacter crescentus

The subunit structure of Caulobacter crescentus chromatin has been proven by electron microscope studies. The use of EDTA-Na2 during the purification of the chromatin complex enhanced the removal of contaminating ribosomes and non-chromatin proteins. The preparation obtained by modified procedure contained RNA polymerase as one of the major proteins and three histone-like proteins (10 K, 17 K and a hitherto not described protein with mol. wt 14 K).