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Sandwich-dot enzyme-linked immunosorbent assay for the detection of canine distemper virus
Zhi Li1, Yanlong Zhang, Huiguo Wang
1Clinical Laboratory, Dalian Municipal Central Hospital, 826-Xinan Road, Shahekou District, Dalian city, Liaoning 116033, China (Zhi Li); Department of Wildlife Medicine, College of Wildlife Resources, Northeast Forestry University, 26-Hexing Road, Harbin, Heilongjiang 150040, China (Zhang); Institute of Immunology, College of Life Science and Technology, Dalian University, 10-Xuefu Avenue, Dalian Economical and Technological Development Zone, Liaoning 116622, China (Wang, Jin, Wenzhe Li).
Abstract:
A sandwich-dot enzyme-linked immunosorbent assay (dot ELISA) was developed for the detection of canine distemper virus (CDV). In 56 dogs suspected to have CD the rates of detection of CDV antigen in samples of blood lymphocytes and palpebral conjunctiva by dot ELISA and ELISA were, respectively, 91% (49/54) and 81% (44/54) for the lymphocyte samples and 88% (28/32) and 75% (24/32) for the conjunctival samples. The CDV detection limits were 10 ng/50 μL for dot ELISA and 40 ng/50 μL for ELISA. The reliability of dot ELISA relative to electron microscopy was 96% with 22 samples: all 21 samples in which CDV particles were observed by electron microscopy yielded positive results with dot ELISA; the single sample in which particles were not observed yielded false-positive results with dot ELISA. The results indicate that the dot ELISA developed can serve as a reliable rapid diagnostic test in suspected cases of CD and also be useful for epidemiologic surveillance of the disease.
