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Updated: Aug 18, 2026

A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium
Published on: February 7, 2016
The formation at 37 C of a nondissociable receptor-estradiol complex
Abstract:
The receptor-estradiol complex formed in rat uterine cytosol when heated at 37 C converts from a dissociable to a nondissociable form. The conversion is best observed in cytosols pretreated with charcoal at 0 C which renders the subsequently formed receptor-estradiol complexes thermostable at 37 C. In the presence of dithiothreitol the heated complex remains dissociable. Tamoxifen does not form nondissociable complexes with the estradiol receptor. It is proposed that the nondissociable form of the receptor complex is a required phase in the mechanism of estradiol action.
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