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A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Published on: March 24, 2017
Efflux inhibition with verapamil potentiates bedaquiline in Mycobacterium tuberculosis
Shashank Gupta1, Keira A Cohen, Kathryn Winglee
1Center for Tuberculosis Research, Department of Medicine, JHU, Baltimore, Maryland, USA.
Abstract:
Drug efflux is an important resistance mechanism in Mycobacterium tuberculosis. We found that verapamil, an efflux inhibitor, profoundly decreases the MIC of bedaquiline and clofazimine to M. tuberculosis by 8- to 16-fold. This exquisite susceptibility was noted among drug-susceptible and drug-resistant clinical isolates. Thus, efflux inhibition is an important sensitizer of bedaquiline and clofazimine, and efflux may emerge as a resistance mechanism to these drugs.
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