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The Rivalta's test as a diagnostic variable in feline effusions--evaluation of optimum reaction and storage
Y Fischer1, K Weber, C Sauter-Louis
1Katrin Hartmann, Medizinische Kleintierklinik, der Ludwig-Maximilians-Universität München, Veterinärstraße 13, 80539 München, Germany,
Objective:
The Rivalta's test is used to diagnose feline infectious peritonitis (FIP) in cats with effusion. Only little information on the influence of sample storage and reaction conditions on test results is available, and diagnostic sensitivity and specificity to diagnose FIP vary considerably between few available studies. This study determined the influence of storage of effusion, modifications on reaction conditions, and inter-observer variation.
Material And Methods:
The Rivalta's test was repeated up to 21 days after storage at room temperature, in the refrigerator, or freezer. The test was performed by two independent, blinded investigators. It was also performed using different volumes of acetic acid, different acids, and different kinds of water.
Results:
Even after storage for 21 days, test results were comparable. While inter-observer variation revealed substantial disagreement, different modifications in performance showed no major influence on test outcome.
Conclusion:
The Rivalta's test seems to be a very robust test concerning storage conditions. Modifications in reaction condition also do not substantially influence outcome. However, the test is subjective and depends on the evaluating person.
