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Related Experiment Video

Updated: May 6, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
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Aptamer-based thrombin assay on microfluidic platform.

Fang-Yuan Chen1, Zheng Wang, Peng Li

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing, P. R. China.

Electrophoresis
|October 16, 2013
PubMed
Summary
This summary is machine-generated.

A new aptamer-based assay on a microfluidic chip offers sensitive detection of thrombin protein. This method optimizes conditions for accurate protein quantification in minimal sample volumes.

Keywords:
AptamerMagnetic beadsMicroarray scannerMicrofluidic chipThrombin

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Protein assays are crucial for disease diagnosis and monitoring.
  • Microfluidic platforms offer advantages in sample handling and analysis speed.
  • Aptamers provide specific molecular recognition for target proteins.

Purpose of the Study:

  • To develop a facile and sensitive aptamer-based protocol for protein assay.
  • To utilize a microfluidic platform with fluorescence detection for enhanced sensitivity.
  • To optimize experimental conditions for accurate thrombin detection.

Main Methods:

  • Aptamer-functionalized magnetic beads were used for thrombin capture.
  • A second, fluorescently labeled aptamer (Cy3) was employed for detection.
  • Experimental parameters including incubation, washing, and interfering proteins were optimized.
  • An off-chip microarray scanner was used for fluorescence detection.

Main Results:

  • A linear relationship was observed between fluorescence intensity and thrombin concentration (65-1000 ng/mL).
  • The assay demonstrated high sensitivity, detecting as low as 0.4 fmol of thrombin.
  • Relative Standard Deviation (RSD) was consistently below 8%.
  • Minimal sample volume (1 μL) was required for analysis.

Conclusions:

  • The developed aptamer-based microfluidic assay is sensitive and facile for protein detection.
  • The method shows excellent performance in quantifying thrombin in complex matrices like human serum.
  • This approach holds promise for clinical diagnostics and biochemical analysis.