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Updated: May 6, 2026

Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice
Published on: March 30, 2016
Evidence of plasticity in the pontocerebellar conditioned stimulus pathway during classical conditioning of the
Jo Anne Tracy1, Judith K Thompson, David J Krupa
1Neuroscience Program, University of Southern California.
Abstract:
Electrical stimulation thresholds required to elicit eyeblinks with either pontine or cerebellar interpositus stimulation were measured before and after classical eyeblink conditioning with paired pontine stimulation (conditioned stimulus, CS) and corneal airpuff (unconditioned stimulus, US). Pontine stimulation thresholds dropped dramatically after training and returned to baseline levels following extinction, whereas interpositus thresholds and input-output functions remained stable across training sessions. Learning rate, magnitude of threshold change, and electrode placements were correlated. Pontine projection patterns to the cerebellum were confirmed with retrograde labeling techniques. These results add to the body of literature suggesting that the pons relays CS information to the cerebellum and provide further evidence of synaptic plasticity in the cerebellar network.
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