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Published on: May 10, 2013
Maillard reaction products as antimicrobial components for packaging films
Carolin Hauser1, Ulla Müller, Tanja Sauer
1Fraunhofer Institute for Process Engineering and Packaging (IVV), Giggenhauser Strasse 35, 85354 Freising, Germany; Department of Chemistry and Pharmacy, Food Chemistry, Emil Fischer Center, Friedrich-Alexander-University of Erlangen-Nuremberg, Schuhstrasse 19, 91052 Erlangen, Germany.
Abstract:
Active packaging foils with incorporated antimicrobial agents release the active ingredient during food storage. Maillard reaction products (MRPs) show antimicrobial activity that is at least partially mediated by H2O2. De novo generation of H2O2 by an MRP fraction, extracted from a ribose/lysine Maillard reaction mixture by 85% ethanol, was monitored at three concentrations (1.6, 16.1, and 32.3g/L) and three temperatures (4, 25, and 37 °C) between 0 and 96 h, reaching a maximum of 335 μM H2O2 (32.3g/L, 37 °C, 96 h). The active MRP fraction (16.1g/L) completely inhibited the growth of Escherichia coli for 24h and was therefore incorporated in a polyvinyl acetate-based lacquer and dispersed onto a low-density polyethylene film. The coated film generated about 100 μM H2O2 and resulted in a log-reduction of >5 log-cycles against E. coli. Thus, MRPs can be considered as active ingredients for antimicrobial packaging materials.
Insights
Maillard reaction products (MRPs) generate hydrogen peroxide (H2O2), showing antimicrobial properties. Incorporating MRPs into active packaging films effectively inhibits bacterial growth, offering a novel food preservation strategy.
Area of Science:
- Food Science
- Materials Science
- Microbiology
Background:
- Active packaging utilizes antimicrobial agents to extend food shelf-life.
- Maillard reaction products (MRPs) exhibit antimicrobial activity, partly due to hydrogen peroxide (H2O2) generation.
Purpose of the Study:
- To investigate the H2O2 generation capacity of an MRP fraction.
- To evaluate the antimicrobial efficacy of MRP-incorporated active packaging films against Escherichia coli.
Main Methods:
- An MRP fraction was extracted and its H2O2 production was monitored under varying concentrations and temperatures.
- The active MRP fraction was incorporated into a polyvinyl acetate lacquer and coated onto low-density polyethylene films.
- Antimicrobial efficacy was assessed by measuring bacterial log-reduction against E. coli.
Main Results:
- The MRP fraction demonstrated de novo H2O2 generation, reaching up to 335 μM.
- An MRP concentration of 16.1 g/L completely inhibited E. coli growth for 24 hours.
- The coated packaging film generated approximately 100 μM H2O2 and achieved a >5 log-cycle reduction of E. coli.
Conclusions:
- MRPs are effective H2O2 generators with significant antimicrobial potential.
- MRP-functionalized active packaging materials show promise for inhibiting bacterial contamination in food products.
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