Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thrombin-mast cell interactions. Binding and cell activation.

E Razin, D Baranes, G Marx

    Experimental Cell Research
    |October 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Short-time mentoring - enhancing female medical students' intentions toward surgical careers.

    Medical education online·2024
    Same author

    [Overtreatment in intensive care medicine-important topics in a challenging time that need a sophisticated dialog].

    Medizinische Klinik, Intensivmedizin und Notfallmedizin·2021
    Same author

    Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm.

    Scientific reports·2021
    Same author

    [S2k Guideline - Recommendations for Inpatient Therapy of Patients with COVID-19].

    Pneumologie (Stuttgart, Germany)·2021
    Same author

    Recommendations for treatment of critically ill patients with COVID-19 : Version 3 S1 guideline.

    Der Anaesthesist·2020
    Same author

    [Perception of the COVID-19 Pandemic among Pneumology Professionals in Germany].

    Pneumologie (Stuttgart, Germany)·2020
    Same journal

    The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

    Experimental cell research·2026
    Same journal

    Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

    Experimental cell research·2026
    Same journal

    Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

    Experimental cell research·2026
    Same journal

    MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

    Experimental cell research·2026
    Same journal

    Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

    Experimental cell research·2026
    Same journal

    Neutrophilic differentiation of promyelocytic HL-60 cells is associated with increased expression of terminal galactose residues on the cell surface.

    Experimental cell research·2026
    See all related articles

    Thrombin activates mouse mast cells to release histamine, but not rat mast cells. Thrombin

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Mast cells play a crucial role in allergic reactions and inflammation.
    • Thrombin is a key enzyme in the coagulation cascade with potential roles beyond hemostasis.

    Purpose of the Study:

    • To investigate the direct effect of thrombin on mast cell activation and histamine release.
    • To determine the binding characteristics and functional domains of thrombin involved in mast cell interaction.

    Main Methods:

    • Activation of mouse bone marrow-derived mast cells (BMMC) and rat peritoneal mast cells (RMC) with varying concentrations of thrombin.
    • Measurement of histamine release.
    • Use of diisopropylfluorophosphate (DFP) and antithrombin III (AT-III) to inhibit thrombin activity.
    • Fluorescently labeled thrombin (FITC-thrombin) for binding studies using fluorescence cytometry.

    Related Experiment Videos

  • Intracellular cyclic AMP (cAMP) measurements.

    • Main Results:

    • Thrombin induced a concentration-dependent histamine release from BMMC, but not RMC.
    • DFP inhibited both thrombin's degranulatory and coagulant activities.
    • FITC-thrombin specifically bound to BMMC, and this binding was blocked by excess unlabeled thrombin.
    • AT-III inhibited thrombin's degranulatory activity and binding to BMMC, while DFP did not affect binding.
    • Thrombin induced a transient increase in intracellular cAMP in BMMC.

    Conclusions:

    • Thrombin directly activates mouse mast cells, leading to histamine release, suggesting a role in inflammatory responses at injury sites.
    • The binding and catalytic regions of thrombin are distinct, with AT-III interfering with binding and DFP affecting catalytic activity.
    • Differential mast cell activation by thrombin may occur in specific physiological contexts like tissue injury.