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Updated: May 6, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
Published on: July 21, 2018
Multi-channel hyperspectral fluorescence detection excited by coupled plasmon-waveguide resonance
1Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. heyh@sz.tsinghua.edu.cn.
We developed a novel biosensor using coupled plasmon-waveguide resonance (CPWR) for sensitive fluorescence detection. This advanced technique achieves a 0.1 nM detection limit and enables multiplex analysis of overlapping fluorescent signals.
Area of Science:
- Biomedical Engineering
- Spectroscopy
- Nanotechnology
Background:
- Fluorescence spectroscopy is crucial for biomolecular detection.
- Existing biosensors face limitations in sensitivity and multiplexing capabilities.
- Coupled plasmon-waveguide resonance (CPWR) offers enhanced light-matter interactions.
Purpose of the Study:
- To develop a highly sensitive biosensor using CPWR-excited fluorescence spectroscopy.
- To investigate optimal sensor parameters for enhanced fluorescence excitation.
- To demonstrate multiplex analysis of overlapping fluorescent analytes.
Main Methods:
- Design and fabrication of a symmetrical CPWR structure.
- Theoretical and experimental investigation of sensor film parameters.
- Hyperspectral fluorescence imaging for spectral recording.
- Detection of Cy5 and Dylight680 fluorescence.
Main Results:
- Achieved a low detection limit of 0.1 nM for Cy5.
- Demonstrated efficient fluorescence excitation via guided waveguide modes.
- Successfully distinguished highly overlapping fluorescence spectra (Cy5 and Dylight680).
- Accurately determined ratios of different emission sources.
Conclusions:
- The developed CPWR biosensor significantly enhances fluorescence detection sensitivity.
- Hyperspectral imaging combined with CPWR enables advanced multiplex analysis.
- This biosensor platform shows great potential for sensitive and multiplexed detection of fluorescence analytes.
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