Related Experiment Video

Updated: May 6, 2026

The Murine Choline-Deficient, Ethionine-Supplemented CDE Diet Model of Chronic Liver Injury
07:27

The Murine Choline-Deficient, Ethionine-Supplemented CDE Diet Model of Chronic Liver Injury

Published on: October 21, 2017

11.2K

Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect

Valeria Denninghoff1, Georgina Ossani, Ana Uceda

  • 1Departamento de Patología, Facultad de Medicina, Centro de Patología Experimental y Aplicada (CPEA), Universidad de Buenos Aires, J. E. Uriburu 950, 5º piso, (C1114AAD), Ciudad Autónoma de Buenos Aires, Argentina, vdenninghoff@cemic.edu.ar.

European Journal of Nutrition
|October 17, 2013
PubMed
Abstract

Insights

Menhaden oil, a type of fish oil, demonstrated protective effects against kidney injury in a rat model. Transcriptomic analysis identified 32 genes potentially involved in this renal protection.

Area of Science:

  • Nutritional biochemistry
  • Renal toxicology
  • Transcriptomics

Background:

  • Kidney injury can result from nutritional imbalances.
  • Fish oil components are investigated for potential health benefits.

Purpose of the Study:

  • To investigate the protective effects of fish oil against kidney damage using transcriptomic data.
  • To identify genes associated with renal protection in a nutritional model.

Main Methods:

  • Wistar rats were fed choline-deficient or choline-supplemented diets with vegetable oil or menhaden oil.
  • Kidney transcriptomic analysis was performed using Affymetrix GeneChip Rat Gene 1.0 ST Array.
  • Differential gene expression analysis was conducted between dietary groups.

Main Results:

  • Rats fed a choline-deficient diet with vegetable oil developed renal cortical necrosis.
  • Menhaden oil groups showed no renal alterations.
  • Analysis between supplemented menhaden oil and supplemented vegetable oil groups revealed 32 differentially expressed genes.

Conclusions:

  • Menhaden oil exhibits a protective effect in this nutritional experimental model.
  • 32 genes, including Gstp1, were identified as potentially responsible for the observed renal protection.
  • Gene expression changes are implicated in the development of renal injury.

More Related Videos

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury07:58

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

Published on: July 17, 2016

8.6K
Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research03:13

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Published on: November 3, 2023

3.3K

Related Concept Videos

Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
2.4K
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
582
Acute Kidney Injury I: Introduction01:22

Acute Kidney Injury I: Introduction

Introduction:Acute Kidney Injury (AKI) describes a swift decrease in kidney function occurring over hours to days, characterized by the kidneys' failure to remove waste products from the bloodstream. This leads to dangerous complications like metabolic acidosis, fluid overload, and electrolyte imbalances, such as hyperkalemia, which can cause life-threatening arrhythmias. AKI is common in both hospital and outpatient settings, often triggered by dehydration, sepsis, or exposure to nephrotoxic...
1.5K
Acute Kidney Injury III: Clinical Manifestations01:29

Acute Kidney Injury III: Clinical Manifestations

Acute Kidney Injury (AKI) progresses through distinct clinical phases: the oliguric, diuretic, and recovery phases, each marked by unique manifestations and challenges.Oliguric Phase:The oliguric phase is the initial stage of AKI, typically lasting 10 to 14 days. This phase is marked by a significant reduction in urine output, usually less than 400 mL per day, indicating decreased kidney function. Fluid retention is a prominent feature, leading to symptoms such as edema, hypertension, and...
1.8K
Acute Pancreatitis II: Pathophysiology01:21

Acute Pancreatitis II: Pathophysiology

The pathophysiology of acute pancreatitis centers on injury to pancreatic acinar cells, which initiates a cascade of harmful intracellular events.This injury leads to premature activation of trypsinogen to trypsin in the pancreas. Trypsin then activates other digestive enzymes, such as chymotrypsin, elastase, and phospholipase A2, which begin breaking down pancreatic tissue. The resulting autodigestion causes local inflammation, tissue swelling, hemorrhage, and fat necrosis.Injured acinar cells...
52
Acute Kidney Injury V: Interprofessional Care01:20

Acute Kidney Injury V: Interprofessional Care

Acute Kidney Injury (AKI) requires a collaborative healthcare approach to restore renal function and prevent complications. Essential management strategies involve monitoring fluid and electrolyte balance, adjusting medications, initiating dialysis when necessary, and providing nutritional support.Fluid and Electrolyte ManagementFluid Monitoring: Regularly monitoring body weight, central venous pressure, and urine output helps detect fluid imbalances early. Patient intake and output are...
548