Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lectin-binding pattern in normal human gastric mucosa. A light and electron microscopic study.

M Ito, K Takata, S Saito

    Histochemistry
    |January 1, 1985
    PubMed
    Summary

    Lectins reveal specific binding patterns in human gastric mucosal cells. This study identifies the Golgi apparatus and secretory canaliculi using lectin probes, enhancing understanding of cell structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hyaluronic acid suppresses the reduction of alpha2(VI) collagen gene expression caused by interleukin-1beta in cultured rabbit articular chondrocytes.

    The Tohoku journal of experimental medicine·1999
    Same author

    Immunohistochemical localization of beta-1,4-galactosyltransferase in human pancreatic tissues.

    Pancreas·1999
    Same author

    Changes in levels of 8-hydroxyguanine in DNA, its repair and OGG1 mRNA in rat lungs after intratracheal administration of diesel exhaust particles.

    Carcinogenesis·1999
    Same author

    Evaluation of serum lipid abnormalities in chronic nephritis.

    Kidney international. Supplement·1999
    Same author

    Distribution of ciprofloxacin into the central nervous system in rats with acute renal or hepatic failure.

    The Journal of pharmacy and pharmacology·1999
    Same author

    Subcellular localization of glucocorticoid receptor protein in the human kidney glomerulus.

    Kidney international·1999

    Area of Science:

    • Gastroenterology
    • Cell Biology
    • Histology

    Background:

    • Human gastric mucosal cells possess complex glycoconjugate structures.
    • Understanding these structures is crucial for diagnosing gastric diseases.

    Purpose of the Study:

    • To investigate the distribution of glycoconjugates in normal human gastric mucosal cells.
    • To characterize specific cellular compartments using lectin probes.

    Main Methods:

    • Light and electron microscopy were employed.
    • Biotinylated and HRP-labeled lectins were used with the ABC method.
    • Specific lectins, including DBA, RCA, and ConA, were utilized.

    Main Results:

    • Lectins demonstrated distinct binding patterns in gastric mucosal cells.

    Related Experiment Videos

  • DBA lectin specifically stained the Golgi apparatus in surface epithelial cells.
  • DBA, RCA, and ConA bound to intracellular secretory canaliculi in parietal cells.

    • Conclusions:

    • Lectins are valuable tools for in situ cytochemical analysis of gastric mucosal glycoconjugates.
    • Specific lectin binding correlates with known cellular organelles like the Golgi apparatus and secretory canaliculi.
    • This research provides detailed insights into the ultrastructural localization of glycoconjugates in the human gastric mucosa.