Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute in vivo proximity labeling for membrane targeted proteomics in neuronal circuits.

bioRxiv : the preprint server for biology·2026
Same author

Development and Validation of a Streamlined Workflow for Proteomic Analysis of Proteins and Post-translational Modifications from Dried Blood.

Journal of proteome research·2026
Same author

Darier disease-A review highlighting new insights from the Darier Disease International Task Force.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same author

Familial hidradenitis suppurativa is associated with an increased prevalence of metabolic and cardiovascular comorbidity.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026
Same author

Defining Histological Patterns in Inherited Ichthyoses: Toward a Diagnostic Algorithm Based on 66 Confirmed Cases.

Dermatopathology (Basel, Switzerland)·2026
Same author

A humanized engineered heart tissue platform for cardiotoxicity assessment.

Stem cell reports·2026

Related Experiment Video

Updated: May 6, 2026

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry
09:18

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Published on: September 5, 2013

23.2K

Distinguishing ichthyoses by protein profiling.

Robert H Rice1, Katie M Bradshaw, Blythe P Durbin-Johnson

  • 1Department of Environmental Toxicology and Forensic Science Graduate Program, University of California Davis, Davis, California, United States of America.

Plos One
|October 17, 2013
PubMed
Summary
This summary is machine-generated.

Protein profiling of skin stratum corneum effectively characterizes different types of ichthyosis. This method distinguishes genetic ichthyosis subtypes and correlates protein changes with disease severity and genetic complexity.

More Related Videos

Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

1.2K
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

13.5K

Related Experiment Videos

Last Updated: May 6, 2026

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry
09:18

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Published on: September 5, 2013

23.2K
Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

1.2K
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

13.5K

Area of Science:

  • Dermatology
  • Proteomics
  • Genetics

Background:

  • Ichthyoses are a group of genetic skin disorders characterized by dry, scaling skin.
  • Accurate characterization of ichthyosis subtypes is crucial for understanding disease mechanisms and developing targeted therapies.
  • Protein profiling offers a potential avenue for detailed molecular phenotyping of these conditions.

Purpose of the Study:

  • To evaluate the utility of shotgun proteomics for characterizing human epidermal stratum corneum.
  • To determine site-specific protein profiles of normal human epidermis.
  • To differentiate protein expression patterns in various ichthyosis subtypes and correlate them with genetic defects.

Main Methods:

  • Shotgun proteomics was employed to analyze protein profiles of human epidermal stratum corneum.
  • Samples were collected from six anatomical sites of healthy individuals.
  • Samples from patients with ichthyosis vulgaris (FLG deficiency), X-linked ichthyosis (STS deficiency), and lamellar ichthyosis (TGM1 deficiency) were analyzed.

Main Results:

  • Significant site-specific differences in protein profiles were observed in normal epidermis.
  • Distinct protein expression patterns were identified for each ichthyosis subtype, clearly distinguishable from normal skin.
  • The degree of protein profile alteration correlated with disease severity, being less pronounced in ichthyosis vulgaris than lamellar ichthyosis.
  • Analysis of familial samples revealed correlations between protein profiles and complex genetic constellations, including modifying gene mutations.

Conclusions:

  • Shotgun proteomics is a valuable tool for the molecular characterization and subtyping of ichthyoses.
  • Protein profiling can reveal distinct molecular signatures for different genetic forms of ichthyosis.
  • This approach holds promise for understanding genotype-phenotype correlations and disease pathogenesis in ichthyosis.