Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

7.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
7.6K
Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness, pharmacokinetics, and safety of triptorelin acetate microspheres in patients with locally advanced and metastatic prostate cancer.

Therapeutic advances in medical oncology·2024
Same author

Effects of vegetation restoration on soil aggregate characteristics and soil erodibility at gully head in Loess hilly and gully region.

Scientific reports·2024
Same author

Predicting Preoperative Risk and Prognosis in Patients with Proliferative Hepatocellular Carcinoma.

Academic radiology·2024
Same author

Similar pipeline experiment and disaster control emergency plan of updraft airflow fire in mine.

Scientific reports·2024
Same author

Effect of AM fungi on the growth and powdery mildew development of Astragalus sinicus L. under water stress.

Plant physiology and biochemistry : PPB·2024
Same author

Fumarate Hydratase-Deficient Renal Cell Carcinoma With Paraganglioma Detected on 18 F-FDG PET/CT.

Clinical nuclear medicine·2024
Same journal

Modeling and analysis of forward and inverse kinematics for a flexible Stewart platform.

PloS one·2026
Same journal

Barriers and facilitators to healthcare utilization amongst people living with sickle cell disease in the United States: A scoping review.

PloS one·2026
Same journal

Enhancing data completeness in time series: Imputation strategies for missing data using significant periodically correlated components.

PloS one·2026
Same journal

Key targets and mechanisms by which gut microbiota-derived metabolites regulate Alzheimer's disease through the immune - inflammatory pathway: Based on network pharmacology and molecular docking.

PloS one·2026
Same journal

Grid-tied Transformer-less Boost Switched Capacitor Topology (TLBSCT) for PV applications.

PloS one·2026
Same journal

The load-velocity profiles and exercise-specific velocity zones for seven commonly used weightlifting exercises.

PloS one·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

19.5K

Long non-coding RNA expression profiling of mouse testis during postnatal development.

Jin Sun1, Yi Lin, Ji Wu

  • 1Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China.

Plos One
|October 17, 2013
PubMed
Summary
This summary is machine-generated.

This study reveals thousands of long non-coding RNAs (lncRNAs) are expressed during mouse testis development. Many lncRNAs are differentially expressed, suggesting crucial roles in male fertility and spermatogenesis.

More Related Videos

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
10:00

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

11.2K
Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

1.8K

Related Experiment Videos

Last Updated: May 6, 2026

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

19.5K
RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
10:00

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

11.2K
Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

1.8K

Area of Science:

  • Reproductive Biology
  • Genomics
  • Molecular Biology

Background:

  • Mammalian testis development and spermatogenesis are vital for male fertility.
  • Research has focused on protein-coding genes and small non-coding RNAs.
  • The role of long non-coding RNAs (lncRNAs) in post-natal testis development is largely unknown.

Purpose of the Study:

  • To investigate lncRNA expression profiles during post-natal mouse testis development.
  • To identify differentially expressed lncRNAs and candidate regulatory lncRNAs.
  • To provide a foundation for understanding lncRNA functions in spermatogenesis.

Main Methods:

  • Microarray analysis of lncRNA expression in neonatal and adult mouse testes.
  • Bioinformatic analysis including genomic context, gene ontology enrichment, and promoter analysis.
  • Assessment of evolutionary conservation of lncRNA elements.

Main Results:

  • 8,265 lncRNAs were expressed, with 3,025 showing differential expression during testis development.
  • Candidate lncRNAs were identified near key spermatogenesis-related genes (e.g., Ovol1, Sox9, c-Kit).
  • Most differentially expressed lncRNAs exhibited epigenetic modifications, tissue-specific expression, and evolutionary conservation.

Conclusions:

  • This is the first systematic investigation of lncRNA expression in mammalian testes.
  • lncRNAs are significantly involved in post-natal testis development and spermatogenesis.
  • Findings establish a basis for future research on lncRNA regulatory mechanisms in male reproduction.