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Related Experiment Videos

Elevated density of [3H]imipramine binding in aged human brain.

J A Severson, J O Marcusson, H H Osterburg

    Journal of Neurochemistry
    |November 1, 1985
    PubMed
    Summary

    Aging increases specific [3H]imipramine binding sites in human brain regions like the hypothalamus and cortex. This age-related change in antidepressant binding sites mirrors findings in aged mouse brains.

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    The journals of gerontology. Series A, Biological sciences and medical sciences·2000

    Area of Science:

    • Neuroscience
    • Gerontology
    • Pharmacology

    Background:

    • Aging impacts neurotransmitter systems, including serotonergic pathways.
    • Previous studies suggest age-related alterations in antidepressant binding sites in animal models.

    Purpose of the Study:

    • To investigate age-related changes in [3H]imipramine binding sites in human postmortem brain tissue.
    • To examine the influence of aging on the in vitro regulation of [3H]imipramine binding by ions.
    • To assess age-related changes in serotonin metabolism.

    Main Methods:

    • Postmortem human brain specimens (hypothalamus, frontal cortex, parietal cortex) were used.
    • Radioligand binding assays with [3H]imipramine were performed.
    • In vitro ion regulation studies (sodium, chloride) and measurement of serotonin and 5-hydroxyindoleacetic acid concentrations were conducted.

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    Main Results:

    • An age-associated increase in [3H]imipramine binding site density was observed in the hypothalamus, frontal cortex, and parietal cortex.
    • In vitro sodium regulation of [3H]imipramine binding was impaired with age in hypothalamic homogenates.
    • Chloride regulation remained intact, and no significant age-related changes in serotonin metabolism were detected.

    Conclusions:

    • Aging increases specific antidepressant binding sites in the human brain.
    • Impaired ion-mediated regulation of these binding sites is associated with aging.
    • These findings in humans parallel observations in aged mouse brains, suggesting conserved aging mechanisms in serotonergic systems.