Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Morphogenesis02:19

Morphogenesis

20.0K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
20.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Control of plant organ growth linked to cell division orientation in inner tissues.

Current biology : CB·2026
Same author

Secure AI-assisted angoff standard-setting for single best answer questions: A non-inferiority validation study.

Medical teacher·2026
Same author

Cell geometry and mechanical stress coordinate stomatal division orientation.

Cell reports·2026
Same author

RNA targeting therapy for a prenatally enriched potassium channel associated with severe childhood epilepsy and premature death.

Nature communications·2026
Same author

Morphogenesis of moss leaf-like organs through variations in deeply shared developmental principles.

Science advances·2026
Same author

Patient-derived pediatric brain tumor orthotopic xenografts and tumor organoids faithfully recapitulate primary tumors.

Science advances·2026

Related Experiment Video

Updated: May 6, 2026

Live Confocal Imaging of Developing Arabidopsis Flowers
07:27

Live Confocal Imaging of Developing Arabidopsis Flowers

Published on: April 1, 2017

16.9K

Quantifying cell shape and gene expression in the shoot apical meristem using MorphoGraphX.

Pierre Barbier de Reuille1, Sarah Robinson, Richard S Smith

  • 1Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Köln, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|October 18, 2013
PubMed
Summary

Confocal microscopy allows live imaging of plant tissues, enabling 3D scanning of cells. This protocol uses MorphoGraphX software to map shoot apex growth and quantify protein expression.

More Related Videos

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
07:52

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Published on: May 23, 2020

4.7K
Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
06:46

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Published on: March 29, 2019

12.3K

Related Experiment Videos

Last Updated: May 6, 2026

Live Confocal Imaging of Developing Arabidopsis Flowers
07:27

Live Confocal Imaging of Developing Arabidopsis Flowers

Published on: April 1, 2017

16.9K
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
07:52

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Published on: May 23, 2020

4.7K
Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
06:46

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Published on: March 29, 2019

12.3K

Area of Science:

  • Plant biology
  • Microscopy
  • Image analysis

Background:

  • Confocal microscopy is a key technique for live imaging of plant tissues.
  • Fluorescent probes targeting cell walls or plasma membranes enable cell visualization.
  • Confocal microscopy offers 3D scanning capabilities with submicron precision.

Purpose of the Study:

  • To present a protocol for extracting surface geometry and cell shapes in the shoot apex.
  • To enable precise growth mapping of the shoot apex using 3D image processing.
  • To facilitate cellular-level quantification of protein expression.

Main Methods:

  • Utilized confocal microscopy for live imaging of plant shoot apices.
  • Employed fluorescent probes to visualize cell walls and plasma membranes.
  • Applied 3D image processing software MorphoGraphX for data analysis and segmentation.

Main Results:

  • Successfully extracted surface geometry and cell shapes from shoot apex images.
  • Generated precise growth maps by segmenting cells over consecutive time points.
  • Demonstrated the capability to tag proteins of interest and quantify their expression.

Conclusions:

  • The presented protocol effectively utilizes confocal microscopy and MorphoGraphX for detailed plant shoot apex analysis.
  • This method provides a powerful tool for studying plant growth dynamics and cellular protein expression.
  • The technique offers high precision for 3D reconstruction and quantitative biological studies.