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Updated: May 6, 2026

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

Published on: July 28, 2011

Optical trapping in plant cells.

Tijs Ketelaar¹, Norbert de Ruijter, Stefan Niehren

¹Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)

|October 18, 2013

Summary

Optical tweezers enable noninvasive manipulation of cellular components for studying physical interactions. This chapter details integrating optical tweezers with confocal microscopy for force measurements in plant cells.

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Optical tweezers are valuable tools for noninvasive manipulation of subcellular compartments.
Understanding physical interactions and attachments within living cells is crucial for biological research.
Quantitative force measurements can provide insights into cellular mechanics.

Purpose of the Study:

To provide practical guidance on setting up optical tweezers experiments.
To address technical considerations for integrating optical tweezers with confocal microscopy.
To describe protocols for trapping intracellular structures in plant cells.

Main Methods:

Utilizing optical tweezers for precise manipulation of subcellular components.
Integrating optical tweezers systems with confocal microscopy.

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Last Updated: May 6, 2026

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

Published on: July 28, 2011

Optical Clearing of Plant Tissues for Fluorescence Imaging

Optical Clearing of Plant Tissues for Fluorescence Imaging

Published on: January 5, 2022

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Measuring displacements and their delays to infer forces.

Main Results:

Detailed practical tips for experimental setup are provided.
Technical considerations for optical tweezers-confocal microscope integration are highlighted.
Working protocols for trapping intracellular structures in plant cells are described.

Conclusions:

This chapter offers a practical guide for researchers using optical tweezers in cell biology.
Successful integration of optical tweezers and confocal microscopy facilitates advanced cellular studies.
The described protocols enable force measurements on intracellular structures in plant cells.