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Updated: May 6, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Distribution of genes encoding tetracycline resistance and aminoglycoside modifying enzymes in Staphylococcus aureus
M Emaneini1, R Bigverdi, D Kalantar
1Department of Microbiology, School of Medicine, Tehran University of Medical Sciences Tehran, Iran.
Abstract:
Staphylococcus aureus (S. aureus) is one of the most common organisms associated with infections among burn patients and has shown a frequent and rapid development of antibiotic resistance. The presence of genes encoding aminoglycoside modifying enzymes (AME) and tetracycline resistance were detected by PCR and multiplex-PCR. Among the 151 S. aureus isolates recovered from the burn patients, 96 (63.6%) were detected to have mecA gene. The rate of tetracycline resistance genes associated with mecA was 61% (92/151). Forty nine isolates (32.4%) contained tetM, 26 (17.2%) possessed only tetK and 21 (13.9%) contained both tetM and tetK. The presence of the aac(6')-Ie-aph(2'')-I gene was determined in 18 isolates, aph(3')-IIIa in 8 isolates, both the aac(6')-Ie-aph(2'')-I, aph(3')-IIIa and the ant(4')-Ia genes in 69 isolates, both aac(6')-Ie-aph(2'')-I and ant(4')-Ia in 6 isolates, and both the aph(3')-IIIa and the ant(4')-Ia genes in 8 isolates. Most of the strains which harboured the mecA gene also contained the tet and AME genes.
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