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An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
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Gene profiling suggests a common evolution of bladder cancer subtypes
Donna E Hansel1, Zhongfa Zhang, David Petillo
1Department of Pathology, University of California at San Diego, 9500 Gilman Drive, MC 0612, La Jolla, CA 92093, USA. dhansel@ucsd.edu.
BMC Medical Genomics
|October 19, 2013
Summary
Bladder squamous cell carcinoma (SCCa) shares many gene expression changes with urothelial carcinoma (UCa), suggesting a closer molecular relationship than previously thought. SCCa also has unique alterations defining its squamous phenotype.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Bladder cancer comprises diverse subtypes like urothelial carcinoma (UCa) and squamous cell carcinoma (SCCa).
- These subtypes, despite distinct characteristics, originate from the urothelial lining and may share precursor findings.
- The molecular relationship between bladder cancer subtypes remains underexplored.
Purpose of the Study:
- To compare gene expression profiles of UCa and SCCa with normal urothelium.
- To identify common and unique molecular alterations in bladder cancer subtypes.
- To elucidate the molecular relationship between UCa and SCCa.
Main Methods:
- Gene expression analysis using Affymetrix GeneChip Human genome arrays.
- Comparison of UCa (n=10), SCCa (n=9), and normal urothelium (n=8).
- Stratification by supervised/unsupervised clustering and fold change analysis.
Main Results:
- UCa showed 155 differentially expressed genes compared to normal urothelium.
- SCCa exhibited 503 differentially expressed genes, many related to squamous morphology.
- 137 genes were commonly dysregulated in both UCa and SCCa; only 18 were unique to UCa.
- Clustering analysis achieved high classification accuracy (96% supervised, 92.6% unsupervised).
Conclusions:
- Bladder SCCa shares significant gene expression changes with UCa.
- SCCa possesses unique molecular alterations that define its squamous phenotype.
- The molecular similarity suggests SCCa is more closely related to UCa than previously hypothesized.
