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Updated: May 6, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Nucleic acid detection using G-quadruplex amplification methodologies.

Benjamin T Roembke¹, Shizuka Nakayama, Herman O Sintim

¹University of Maryland, College Park, MD 20742, United States.

Methods (San Diego, Calif.)

|October 19, 2013

Summary

This summary is machine-generated.

G-quadruplexes are increasingly used as labels for detecting nucleic acids and other analytes. This review covers strategies using G-quadruplexes for sensitive detection, including various probe designs and signal amplification methods.

Keywords:

Amplification Diagnostics Fluorescence G-quadruplex G-quadruplex–hemin conjugate Gene detection Peroxidase Split G-quadruplex

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Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

G-quadruplexes are unique nucleic acid structures with diverse applications.
Their use as labels for analyte detection has surged in the past decade.

Purpose of the Study:

To review strategies for nucleic acid detection using G-quadruplexes as labels or signal amplifiers.
To briefly mention methods for detecting other analytes.

Main Methods:

Focus on G-quadruplex-based detection strategies: split G-quadruplex, hemin-G-quadruplex conjugates, molecular beacon G-quadruplex, and inhibited G-quadruplex probes.
Discuss tandem use with DNA-modifying enzymes (polymerases, exonucleases, endonucleases) for signal amplification.
Cover various detection modalities: fluorescence, colorimetric, chemiluminescence, and electrochemical methods.

Main Results:

G-quadruplex labels offer versatile platforms for sensitive analyte detection.
Enzyme-mediated signal amplification enhances detection capabilities.
Diverse probe designs and detection methods cater to various analytical needs.

Conclusions:

G-quadruplexes are powerful tools for sensitive and versatile detection of nucleic acids and other analytes.
Integration with enzymatic amplification and various detection modalities expands their utility.
This review highlights key strategies and future directions in G-quadruplex-based sensing.