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Updated: May 6, 2026
Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
Published on: May 27, 2016
Association between clinical pathology and multiple genes mRNA expression in Chinese patients with NSCLC
Gang Chen1, G U Jundong, Jun Chen
1Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin, 300052, China.
Objective:
The aim of this study was to evaluate whether there was an association between pathology type and ERCC1, BRCA1, RRM1, TUBB3, STMN1, TOP2A and epidermal growth factor receptor (EGFR) messenger ribonucleic acid (mRNA) expression level in Chinese patients with non-small cell lung carcinoma (NSCLC).
Materials And Methods:
mRNA expression level of these genes was analyzed in 181 cancer tissues by using xTAG-step liquid-chip array. The mRNA expression level of the seven genes was evaluated in association with the clinical pathology type.
Results:
The average mRNA expression level of the seven genes were ERCC1 (1.02 ± 0.03), BRCA1 (0.15 ± 0.04), RRM1 (0.19 ± 0.05), TUBB3 (0.31 ± 0.06), STMN1 (2.78 ± 0.42), TOP2A (3.04 ± 0.42) and EGFR (0.58 ± 0.09), respectively in Chinese patients with NSCLC. The mRNA expression level of ERCC1, STMN1 and TOP2A genes were statistical different with different pathology type (p(a) < 0.05); STMN1 and TOP2A genes mRNA expression were much higher in squamous cell lung carcinoma than that in non-squamous cell lung carcinoma (p(a) < 0.05). And ERCC1 gene expression was much lower in squamous cell carcinoma than that in non-squamous cell carcinoma (p(a) < 0.05).
Conclusion:
mRNA expression level of STMN1, TOP2A and ERCC1 were correlated with the clinical pathology type.
Insights
Messenger RNA (mRNA) expression levels of STMN1, TOP2A, and ERCC1 genes correlate with pathology type in Chinese patients with non-small cell lung carcinoma (NSCLC). This finding may aid in understanding NSCLC subtypes.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Non-small cell lung carcinoma (NSCLC) is a major cause of cancer-related mortality worldwide.
- Understanding the molecular differences between NSCLC subtypes is crucial for targeted therapy development.
- Gene expression profiling can reveal molecular distinctions relevant to tumor behavior and patient outcomes.
Purpose of the Study:
- To investigate the association between messenger RNA (mRNA) expression levels of seven key genes (ERCC1, BRCA1, RRM1, TUBB3, STMN1, TOP2A, and EGFR) and the pathology type in Chinese patients diagnosed with NSCLC.
- To determine if specific gene expression patterns are linked to different histological subtypes of NSCLC.
Main Methods:
- Analysis of mRNA expression levels for ERCC1, BRCA1, RRM1, TUBB3, STMN1, TOP2A, and EGFR in 181 NSCLC tumor tissues.
- Utilized the xTAG-step liquid-chip array technology for quantitative mRNA analysis.
- Correlated the measured mRNA expression levels with the clinical pathology types of the NSCLC samples.
Main Results:
- Significant differences in mRNA expression levels were observed for ERCC1, STMN1, and TOP2A genes across different NSCLC pathology types (p < 0.05).
- STMN1 and TOP2A mRNA expression levels were significantly higher in squamous cell lung carcinoma compared to non-squamous cell types.
- ERCC1 mRNA expression levels were significantly lower in squamous cell carcinoma relative to non-squamous cell carcinoma.
Conclusions:
- The mRNA expression levels of STMN1, TOP2A, and ERCC1 are significantly correlated with the clinical pathology type in Chinese NSCLC patients.
- These specific gene expression patterns may serve as molecular markers for differentiating NSCLC subtypes, potentially informing treatment strategies.
