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Updated: May 6, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Published on: August 17, 2015
Mass Western for absolute quantification of target proteins and considerations about the instrument of choice
David Lyon1, Wolfram Weckwerth, Stefanie Wienkoop
1Department of Molecular Systems Biology, University of Vienna, Vienna, Austria.
Abstract:
The Mass Western describes the absolute quantification of proteins based on stable isotope labeled integral standard peptides and liquid chromatography coupled selective reaction monitoring triple quadrupole mass spectrometry (LC-SRM/MS). Here, we present a detailed workflow including tips and we discuss advantages and disadvantages of using different types of MS for absolute quantification.
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