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Updated: May 6, 2026

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
Published on: December 22, 2020
Mass spectrometry-based imaging of metabolites and proteins
Manuela Peukert1, Michael Becker, Andrea Matros
1Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Abstract:
Imaging techniques based on mass spectrometry (MS) have become powerful approaches to decipher the spatial distribution of metabolites and proteins. MS imaging (MSI) mostly relies on matrix-assisted laser desorption/ionization coupled to MS detection, but desorption electrospray ionization is also frequently used. Here we describe our current protocols for MALDI-MSI of seed sections and for root tissue. Detailed procedures for cryo-sectioning, matrix application, image capture, mass spectrometry measurement and data analysis are given.
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