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Updated: May 6, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS
Daisuke Takahashi1, Bin Li, Takato Nakayama
1United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate, Japan.
Abstract:
Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for proteins from the plasma membrane proteins and plasma membrane microdomain fraction is described. The results obtained are easily applicable to label-free protein semiquantification.

