Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.1K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.1K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

96.6K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
96.6K
Southern Blot02:57

Southern Blot

15.1K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
15.1K
SDS-PAGE01:27

SDS-PAGE

23.3K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
23.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Importance of Riboswitch-regulated Genes for Pseudomonas aeruginosa.

Journal of molecular biology·2026
Same author

The Thiamin Pyrophosphate Riboswitch is Affected by Lysine-derived Metabolic Conversion Products in Escherichia coli.

Journal of molecular biology·2025
Same author

Insights into the cotranscriptional and translational control mechanisms of the Escherichia coli tbpA thiamin pyrophosphate riboswitch.

Communications biology·2024
Same author

The <i>Escherichia coli ribB</i> riboswitch senses flavin mononucleotide within a defined transcriptional window.

RNA (New York, N.Y.)·2024
Same author

Cotranscriptional Folding of a 5' Stem-loop in the Escherichia coli tbpA Riboswitch at Single-nucleotide Resolution.

Journal of molecular biology·2024
Same author

Structural Characterization of the Cotranscriptional Folding of the Thiamin Pyrophosphate Sensing <i>thiC</i> Riboswitch in <i>Escherichia coli</i>.

Biochemistry·2024

Related Experiment Video

Updated: May 6, 2026

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
08:46

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Published on: August 26, 2020

4.7K

RNA conformational changes analyzed by comparative gel electrophoresis.

Sébastien H Eschbach1, Daniel A Lafontaine

  • 1Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke, Sherbrooke, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2013
PubMed
Summary

Native gel electrophoresis reveals precise details about biologically important RNA structures in solution. This technique offers insights into RNA folding, including dihedral angles and helical rotation, exemplified by studying the SAM riboswitch.

More Related Videos

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

11.3K
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
08:03

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function

Published on: June 21, 2017

9.6K

Related Experiment Videos

Last Updated: May 6, 2026

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
08:46

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Published on: August 26, 2020

4.7K
CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

11.3K
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
08:03

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function

Published on: June 21, 2017

9.6K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Understanding native RNA structures is crucial for elucidating their cellular functions.
  • Native gel electrophoresis is a powerful technique for analyzing RNA structures in solution under varying experimental conditions.

Purpose of the Study:

  • To demonstrate the utility of native gel electrophoresis for detailed RNA structural analysis.
  • To investigate the folding mechanisms of RNA molecules, specifically the S-adenosylmethionine (SAM) sensing riboswitch.

Main Methods:

  • Comparative native gel electrophoresis to determine relative angles between RNA stems.
  • Analysis of structural parameters like dihedral angles and helical rotation.
  • Application of the method to study the folding of the SAM sensing riboswitch.

Main Results:

  • Native gel electrophoresis provides precise information on the relative angles between stems in RNA molecules.
  • The technique allows for the determination of specific structural features, including dihedral angles and helical rotation.
  • The folding of the SAM sensing riboswitch was successfully studied using this electrophoretic approach.

Conclusions:

  • Native gel electrophoresis is a valuable tool for obtaining high-resolution structural information about native RNA molecules in solution.
  • This method offers a quantitative approach to understanding RNA folding and conformational dynamics.
  • The study highlights the potential of native gel electrophoresis for characterizing complex RNA structures and their functions.