Integrin-mediated osteoblastic adhesion on a porous manganese-incorporated TiO₂ coating prepared by plasma...

This study was conducted to evaluate the bioactivity of manganese-incorporated TiO₂ (Mn-TiO₂) coating prepared on titanium (Ti) plate by plasma electrolytic oxidation (PEO) technique in Ca-, P- and Mn-containing electrolytes. The surface topography, phase and element compositions of the coatings were investigated using scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectrometry (EDS), respectively. The adhesion of osteoblast-like MG63 cells onto Ti, TiO₂ and Mn-TiO₂ surfaces was evaluated, and the signal transduction pathway involved was confirmed by the sequential expression of the genes for integrins β₁, β₃, α₁ and α₃, focal adhesion kinase (FAK), and the extracellular regulated kinases (ERKs), including ERK1 and ERK2. The results obtained indicated that Mn was successfully incorporated into the porous nanostructured TiO₂ coating, and did not alter the surface topography or the phase composition of the coating. The adhesion of the MG63 cells onto the Mn-incorporated TiO₂ coating was significantly enhanced compared with that on the Mn-free TiO₂ coating and the pure Ti plates. In addition, the enhanced cell adhesion on the Mn-TiO₂ coatings may have been mediated by the binding of the integrin subunits, β₁ and α₁, and the subsequent signal transduction pathway, involving FAK and ERK2. The study indicated that the novel Mn-TiO₂ coating has potential for orthopedic implant applications, and that further investigations are required.