Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

cDNA clones encoding bovine interphotoreceptor retinoid binding protein.

D J Barrett, T M Redmond, B Wiggert

    Biochemical and Biophysical Research Communications
    |September 30, 1985
    PubMed
    Summary

    Researchers isolated cDNA clones for bovine interphotoreceptor retinoid-binding protein (IRBP). The deduced amino acid sequence matched authentic IRBP, confirming the clones encode this crucial retinal protein.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Seroprevalence of Mycoplasma bovis in bulk milk samples in Irish dairy herds and risk factors associated with herd seropositive status.

    Journal of dairy science·2022
    Same author

    Significant re-emergence and recirculation of Schmallenberg virus in previously exposed dairy herds in Ireland in 2016.

    Transboundary and emerging diseases·2017
    Same author

    Exposure to Schmallenberg virus in Irish sheep in 2013.

    The Veterinary record·2015
    Same author

    Offsetting the impacts of mining to achieve no net loss of native vegetation.

    Conservation biology : the journal of the Society for Conservation Biology·2014
    Same author

    Alternative routes of immunization for the induction of experimental autoimmune uveoretinitis (EAU) in rodents: a comparison.

    Ocular immunology and inflammation·2012
    Same author

    Risk factors associated with Johne's disease test status in dairy herds in Ireland.

    The Veterinary record·2011

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Ophthalmology

    Background:

    • Interphotoreceptor retinoid-binding protein (IRBP) plays a vital role in the visual cycle.
    • Understanding IRBP's structure is crucial for studying retinal function and disease.

    Purpose of the Study:

    • To isolate and characterize cDNA clones encoding bovine interphotoreceptor retinoid-binding protein (IRBP).
    • To confirm the identity of the isolated cDNA clones through sequence analysis.

    Main Methods:

    • Immunological screening of a bovine retinal cDNA expression library using lambda gt11.
    • Hybridization screening of a lambda gt10 cDNA library with a cDNA fragment.
    • Restriction endonuclease mapping.
    • Amino acid sequencing of a tryptic peptide from authentic IRBP.

    Related Experiment Videos

  • Comparison of deduced amino acid sequence from cDNA with authentic peptide sequence.

    • Main Results:

    • Isolation of three cDNA clones (lambda IRBP-1, lambda IRBP-2, lambda IRBP-3) for bovine IRBP.
    • Identification of a single bovine retinal mRNA species of approximately 8 kb.
    • Confirmation that the deduced amino acid sequence from the cDNA matches the authentic IRBP tryptic peptide sequence.

    Conclusions:

    • The isolated cDNA clones definitively encode bovine interphotoreceptor retinoid-binding protein (IRBP).
    • This provides a molecular tool for further investigation into IRBP function and the visual cycle.