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Isotope fractionation and spectroscopic analysis as an evidence of Cr(VI) reduction during biosorption
Hana Šillerová1, Vladislav Chrastný, Eva Čadková
1Department of Environmental Geosciences, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, Praha 6 - Suchdol, 165 21, Czech Republic.
Abstract:
This work investigates the mechanisms behind Cr(VI) biosorption/reduction on three biomaterials (brewers draff, grape waste and synthetic humic acid). Coupled Cr isotope analysis with ICP-OES, XPS and SEM was tested as a novel approach to study the reduction of Cr(VI) by the biomaterials. The Cr(VI) biosorption process was accompanied with heavier Cr isotopes enrichment in the remaining Cr(VI) fraction. A significant fractionation of Cr stable isotopes was observed with no significant pH effect; δ(53)Cr of the remaining fraction ranged from 0.2‰ to 1.9‰ while δ(53)Cr of the product (sorbed Cr) ranged from -1.2‰ to -2.8‰. The Rayleigh fractionation model fitted well the measured data and Cr isotope analysis provides thus an efficient tool to quantify Cr(VI) reduction by different biomaterials. In general, the sorption/reduction potential of the three studied biomaterials decreased in the following order: grape waste>humic acids>brewers draff.
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