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Polygalacturonases from Moniliophthora perniciosa are regulated by fermentable carbon sources and possible

Heliana Argôlo Santos Carvalho1, Edson Mario de Andrade Silva, Stenio Carvalho Santos

  • 1Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus-BA, Brazil.

Fungal Genetics and Biology : FG & B
|October 22, 2013
PubMed

Insights

This study reveals how Monilophthora perniciosa polygalacturonase (PG) genes are regulated by carbon sources and interact with other proteins. These findings offer insights into fungal development and disease management strategies.

Area of Science:

  • * Molecular biology
  • * Mycology
  • * Plant pathology

Background:

  • * Monilophthora perniciosa causes significant crop losses.
  • * Polygalacturonases (PGs) are key enzymes in fungal pathogenesis.
  • * Understanding PG gene regulation is crucial for disease control.

Purpose of the Study:

  • * To perform the first molecular and in silico analysis of Monilophthora perniciosa polygalacturonases (PGs).
  • * To investigate the transcriptional regulation of three MpPG genes under different carbon sources.
  • * To explore protein interaction networks involved in fungal necrotrophic phase.

Main Methods:

  • * Identification and transcriptional analysis of three MpPG genes (MpPG1, MpPG2, MpPG3) using RT-qPCR.
  • * Culturing M. perniciosa mycelium on various solid and liquid media with different carbon sources.
  • * Systems biology analysis integrating gene expression data with orthologs in Neurospora crassa.

Main Results:

  • * Three MpPG genes exhibited distinct expression patterns, primarily regulated by fermentable carbon sources (galactose, mannose).
  • * In silico analysis revealed interactions of MpPG1 and MpPG2 orthologs with proteins involved in protein synthesis, post-translational modifications, cell wall, and energy metabolism.
  • * Key proteins like MpPG2, pectin methylesterase, acetolactate synthase, and SMT3-like were identified for further study.

Conclusions:

  • * Fermentable carbon sources significantly regulate MpPG gene expression in M. perniciosa.
  • * Protein interaction networks highlight potential mechanisms of PG regulation during necrotrophic growth.
  • * Identified proteins offer targets for M. perniciosa disease management and understanding fungal development.
Keywords:
Acetolactate synthaseBCAAEnergy metabolismGOMMMOXORFPGPLPMEPPIPTMPectin methylesteraseSmall ubiquitin-like modifierSystems biologyWitches’ broom diseasebranched chain amino acidgene ontologymethanol oxidasemineral mediumopen reading framepectate lyasepectin methylesterasepolygalacturonasepost-translational modificationprotein–protein interaction

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