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A path forward for the chlamydial virulence factor CPAF

Turner A Conrad1, Zhangsheng Yang, David Ojcius

  • 1Department of Microbiology and Immunology, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, USA.

Microbes and Infection
|October 22, 2013
PubMed

Insights

Chlamydia protease CPAF

Area of Science:

  • Microbiology
  • Pathogenesis
  • Bacterial Proteases

Background:

  • Chlamydia protease CPAF is secreted by Chlamydiales bacteria.
  • Previous studies identified host targets of CPAF.
  • Concerns exist regarding the accuracy of prior experimental methods.

Purpose of the Study:

  • To review the characteristics of CPAF.
  • To propose novel methods for CPAF target validation.
  • To re-evaluate CPAF's role in chlamydial infections.

Main Methods:

  • Literature review of CPAF research.
  • Critical analysis of experimental methodologies.
  • Proposal of new experimental strategies.

Main Results:

  • Many previously identified CPAF host targets may be artifacts.
  • Existing methods for studying CPAF require refinement.
  • A need exists for validated approaches to study CPAF.

Conclusions:

  • The precise function of CPAF in pathogenesis is uncertain.
  • New, accurate methods are essential for understanding CPAF.
  • Further research is needed to elucidate CPAF's true role.
Keywords:
CPAFChlamydial virulence factors and pathogenesisSubstrates

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