Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

854
The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic...
854
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

1.1K
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
1.1K
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

81
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
81
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

7.3K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
7.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

14.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IFNγ-producing iNKTs restrict a live-attenuated chlamydia oral vaccine in the large intestine.

Frontiers in immunology·2026
Same author

Chlamydial histone homologs control developmental fitness in the next infection cycle.

mSphere·2026
Same author

Inhibition of a live-attenuated chlamydia oral vaccine in the large intestine is dependent on CD11c-expressing cells that produce IL-23.

Scientific reports·2026
Same author

Immunological evaluation of vaccines to prevent Chlamydia trachomatis infection using the human in vitro MIMIC system.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

The oral vaccine intrOv is attenuated in infecting the lower airway and inducing chronic pathology.

Infection and immunity·2026
Same author

An All-in-One-Integrated Self-Powered Wearable Sensing System for Multimodal Health Monitoring.

ACS nano·2026

Related Experiment Video

Updated: May 6, 2026

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

3.5K

A path forward for the chlamydial virulence factor CPAF.

Turner A Conrad1, Zhangsheng Yang, David Ojcius

  • 1Department of Microbiology and Immunology, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, USA.

Microbes and Infection
|October 22, 2013
PubMed
Summary

Chlamydia protease CPAF

Keywords:
CPAFChlamydial virulence factors and pathogenesisSubstrates

More Related Videos

Forward Genetic Approaches in Chlamydia trachomatis
09:03

Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

12.4K
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
10:35

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Published on: January 30, 2020

6.4K

Related Experiment Videos

Last Updated: May 6, 2026

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

3.5K
Forward Genetic Approaches in Chlamydia trachomatis
09:03

Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

12.4K
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
10:35

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Published on: January 30, 2020

6.4K

Area of Science:

  • Microbiology
  • Pathogenesis
  • Bacterial Proteases

Background:

  • Chlamydia protease CPAF is secreted by Chlamydiales bacteria.
  • Previous studies identified host targets of CPAF.
  • Concerns exist regarding the accuracy of prior experimental methods.

Purpose of the Study:

  • To review the characteristics of CPAF.
  • To propose novel methods for CPAF target validation.
  • To re-evaluate CPAF's role in chlamydial infections.

Main Methods:

  • Literature review of CPAF research.
  • Critical analysis of experimental methodologies.
  • Proposal of new experimental strategies.

Main Results:

  • Many previously identified CPAF host targets may be artifacts.
  • Existing methods for studying CPAF require refinement.
  • A need exists for validated approaches to study CPAF.

Conclusions:

  • The precise function of CPAF in pathogenesis is uncertain.
  • New, accurate methods are essential for understanding CPAF.
  • Further research is needed to elucidate CPAF's true role.