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Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Yeast Signaling01:28

Yeast Signaling

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Related Experiment Video

Updated: May 6, 2026

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
09:22

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Published on: October 30, 2013

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The one hour yeast proteome.

Alexander S Hebert1, Alicia L Richards, Derek J Bailey

  • 1Departments of Biomolecular Chemistry;

Molecular & Cellular Proteomics : MCP
|October 22, 2013
PubMed
Summary
This summary is machine-generated.

Rapid yeast proteome analysis is now possible in just over one hour using an optimized Orbitrap Fusion mass spectrometer. This breakthrough in proteomics enables faster characterization of complex biological samples.

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Yeast Biology

Background:

  • Comprehensive proteome analysis is crucial for understanding cellular function.
  • Previous methods required extensive time for proteome characterization.

Purpose of the Study:

  • To develop and validate an expedited method for comprehensive yeast proteome analysis.
  • To evaluate the performance of a new Orbitrap hybrid mass spectrometer (Q-OT-qIT) for rapid proteomics.

Main Methods:

  • Utilized optimized sample preparation and chromatographic separation techniques.
  • Employed a high-performance Orbitrap Fusion mass spectrometer (Q-OT-qIT) with advanced acquisition capabilities.
  • Implemented fast MS(2) acquisition (20 Hz) for efficient peptide sequencing.

Main Results:

  • Achieved comprehensive yeast proteome characterization in just over one hour.
  • Detected an average of 3,977 proteins at a 1% false discovery rate (FDR) per hour.
  • Generated high numbers of MS(1) and MS(2) scans, peptide spectral matches, and unique peptide sequences.

Conclusions:

  • The optimized Q-OT-qIT system enables rapid and comprehensive proteome analysis.
  • Further increases in mass spectrometer scan speed could facilitate proteome analysis within hours.
  • This approach has significant implications for accelerating biological research and biomarker discovery.