β-cyclodextrin assistant flavonoid glycosides enzymatic hydrolysis

Area of Science:

• Pharmacology
• Biochemistry
• Materials Science

Background:

• Low content of icaritin and genistein in herba necessitates efficient preparation methods for pharmacological studies.
• Developing scalable and effective extraction/preparation techniques is crucial for utilizing these compounds.

Purpose of the Study:

• To prepare flavonoid glycosides /β-cyclodextrin inclusion complexes.
• To enhance the enzymatic hydrolysis rate of these complexes.
• To optimize conditions for preparing icaritin and genistein.

Main Methods:

• Preparation of flavonoid glycosides /β-cyclodextrin inclusion complexes.
• Differential scanning calorimetry (DSC) for physical property analysis.
• Mono-factor experimental design to optimize enzymatic hydrolysis conditions.

Main Results:

• Solubility of icaritin and genistein increased significantly (17-fold and 28-fold, respectively).
• Enzymatic hydrolysis reaction time decreased by 68% for icaritin and 145% for genistein.
• High purity icaritin (99.34%) and genistein (99.46%) were obtained.

Conclusions:

• The study presents a novel approach to accelerate enzyme-hydrolysis of poorly water-soluble flavonoid glycosides.
• Optimized conditions using β-cyclodextrin inclusion complexes improve the preparation of icaritin and genistein.
• This method offers a superior strategy for obtaining these valuable compounds.