Hematological parameters and redox balance of rat blood in the dynamics of immobilization

Hematological and biochemical parameters were studied in rat blood in the dynamics of immobilization (0.5, 1, and 3 h). Immobilization was followed by a release of old erythrocytes in the circulation and the development of reticulocytosis. The neutrophil count significantly increased by the third hour of immobilization. Restraint stress induced changes in activities of antioxidant enzymes (SOD and catalase) in erythrocytes and plasma level of MDA. The correlation between each enzyme (SOD, glutathione peroxidase, and catalase) and MDA concentration was the highest 1 h after the beginning of immobilization. These data can reflect the tension of antioxidant systems in red blood cells. The biochemical parameters returned to the basal level by the third hour of immobilization. Correlation coefficients between the activities of antioxidant enzymes and MDA amount decreased after 3 h of stress and the correlation between glutathione peroxidase and reduced glutathione significantly increased. The redox status of the blood returned to the initial values after 3 h.