Related Experiment Video
Updated: May 6, 2026
Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection
Published on: June 23, 2023
Effect of ERBB2 expression on invasiveness of glioma TJ905 cells
1Department of Neurosurgery, First Hospital of Xi'an, Jiaotong University, Xi'an 710061, China.
Objective:
To investigate the influence and possible mechanism of ERBB2 expression on the invasiveness of glioma cells.
Methods:
Glioma TJ905 cells were separated and cultured. ERBB2 shRNA and overexpressing vectors were constructed, which were then transfected. The ERBB2 expression was up-regulated or down-regulated. Changes of invasiveness of TJ905 cells were detected by Transwell assay, and the expressions of matrix metalloprotease (MMP)-2 and MMP-9 were measured by Western blot.
Results:
ERBB2 shRNA transfection vector could effectively inhibit expression of ERBB2; while ERBB2 overexpressing vector transfection could significantly improve the expression of ERBB2 in TJ905 cells. Transwell assay showed that when ERBB2 expression was down-regulated, the invasiveness of TJ905 cells was notably decreased; when ERBB2 expression was up-regulated, the invasiveness of TJ905 cells was markedly increased. Meanwhile, Western blot indicated that down-regulating ERBB2 inhibited the expression of MMP-2 and MMP-9, while up-regulating ERBB2 enhanced their expressions.
Conclusions:
ERBB2 expression is closely related to the invasiveness of glioma TJ905 cells.
Insights
Epidermal growth factor receptor 2 (ERBB2) expression significantly impacts glioma cell invasiveness. Modulating ERBB2 levels affects cell migration and the expression of matrix metalloproteinases (MMP)-2 and MMP-9.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cell Biology
Background:
- Glioma is a primary brain tumor with significant invasiveness.
- The role of ERBB2 in glioma cell invasion requires further elucidation.
Purpose of the Study:
- To investigate the influence of ERBB2 expression on glioma cell invasiveness.
- To explore the underlying mechanism involving matrix metalloproteinases (MMP).
Main Methods:
- Cultured human glioma TJ905 cells.
- Utilized ERBB2 shRNA and overexpressing vectors for gene manipulation.
- Assessed cell invasiveness using Transwell assays.
- Quantified MMP-2 and MMP-9 expression via Western blot.
Main Results:
- Down-regulating ERBB2 significantly decreased glioma cell invasiveness.
- Up-regulating ERBB2 markedly increased glioma cell invasiveness.
- ERBB2 expression levels correlated directly with MMP-2 and MMP-9 expression.
Conclusions:
- ERBB2 expression is a key determinant of glioma cell invasiveness.
- ERBB2 influences glioma cell invasion potentially through regulating MMP-2 and MMP-9.
