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Aptamer-based array electrodes for quantitative interferon-γ detection.

Yu Chen1, Tze Sian Pui, Patthara Kongsuphol

  • 1Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research) Singapore, 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.

Biosensors & Bioelectronics
|October 23, 2013
PubMed
Summary

This study presents a novel biosensor for detecting interferon-gamma (IFN-γ). The methylene blue-tagged aptamer on a gold micro-array offers a sensitive and selective method for IFN-γ quantification.

Keywords:
AptamerBiosensorIFN-γMicro-electrode arraySquare Wave Voltammetry

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Interferon-gamma (IFN-γ) is a critical cytokine in immune response.
  • Accurate detection of IFN-γ is essential for diagnosing and monitoring various diseases.
  • Existing detection methods may lack sensitivity, specificity, or require complex procedures.

Purpose of the Study:

  • To develop and characterize a methylene blue-tagged thiolated aptamer-modified gold micro-array based biosensor.
  • To achieve specific and sensitive detection of interferon-gamma (IFN-γ).
  • To evaluate the biosensor's performance, including detection limit and selectivity.

Main Methods:

  • Fabrication of microelectrode arrays using silicon microfabrication.
  • Modification of electrodes with methylene blue-tagged aptamer via gold thiol chemistry.
  • Electrochemical characterization using Cyclic Voltammetry (CV) and Square Wave Voltammetry (SQW).

Main Results:

  • Estimated aptamer density of 4.4 × 10^12 molecules/cm^2 on the modified electrode.
  • Oxidation peak current decreased with increasing IFN-γ concentration, showing signal suppression.
  • Achieved a detection limit of 1.3 ng/ml (0.8 fmol) for IFN-γ in the range of 1-500 ng/ml.
  • Demonstrated negligible signal changes (<5%) in the presence of high non-specific protein (BSA).

Conclusions:

  • The developed aptamer-modified gold micro-array biosensor enables specific and sensitive detection of IFN-γ.
  • The biosensor exhibits high selectivity, effectively minimizing interference from non-specific protein binding.
  • This platform holds promise for accurate diagnostics and monitoring applications involving IFN-γ.